Sensor-integrated proteome-on-chip biosensor platform providing simultaneous quantitative, qualitative, and kinetic proteomic data in a single high-throughput assay for drug and vaccine development, protein biomarker discovery, and diagnostics. Enables thousands of full-length folded proteins per chip via rapid cell-free human lysate expression and on-chip capture for large-scale SPR and mass-spectrometry analyses with greatly reduced time and cost.